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| Pacheco Contreras,Víctor I; Lourenco Jaramillo,Diana L; Parra Bracamonte,Gaspar M; Martínez González,Juan C; Sifuentes Rincón,Ana M. |
| K-casein gene polymorphisms are of major importance in the dairy industry due to their association with different quality and productive traits (i.e., milk protein). Several methods for genotyping this gene have been proposed; however, none are focused on the simultaneous discrimination of nine K-casein variants. A strategy based on PCR-RFLP was designed to characterize nine K-casein variants (A, B, C, E, F², G, H, I and J) and used to genotype three cattle populations: Gyrholando (3/8 Gyr x 5/8 Holstein), Charolais and Carora. The B variant was the most frequent in the Charolais and Carora breeds, with allelic frequencies of 0.60 and 0.59, respectively. In the Gyrholando breed, four variants were found, with the A variant being the most frequent and E and... |
| Tipo: Journal article |
Palavras-chave: Milk proteins; Milk quality; Protein genes. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000400012 |
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| Soares,Maria Amélia Menck; Rodrigues,Marcelo Teixeira; Mognol,Giuliana Patrícia; Ribeiro,Lucinéia de Fátima Chasko; Silva,José Luis da Conceição; Brancalhão,Rose Meire Costa. |
| Three different regions of the alpha s1-casein gene (CSN1S1) were investigated to determine the frequencies of major alleles for null, low, intermediate and high milk protein expression in a herd of dairy goats raised in the southeastern region of Brazil. Genomic DNA samples were obtained from leukocytes of 145 dairy goats and regions of interest in the gene were amplified through Polymerase Chain Reaction (PCR), then evaluated in both agarose (O and E allele) and polyacrylamide gels (F allele). For better characterization of the F allele, a PCR-RFLP (PCR-Restriction Fragment Length Polymorphism) study was performed employing the endonuclease XmnI. The allelic frequencies in the herd of 62 Saanen goats studied were: CSN1S1E = 0.35; CSN1S1F = 0.30; CSN1S1O1... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Alpine; Capra hircus; CSN1S1; Genotyping; Milk proteins; Saanen. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982009000600008 |
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